Micro open-sandwich ELISA to rapidly evaluate thyroid hormone concentration from serum samples
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Updated: Jun 6, 2026
Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model
Published on: March 17, 2023
Kamrun Nahar Islam1, Masaki Ihara, Jinhua Dong
1Department of Chemistry & Biotechnology, University of Tokyo, Tokyo, Japan.
Background:
Thyroxine (T4) is the most commonly measured thyroid hormone for the diagnosis of thyroid function. To elucidate a rapid and sensitive assay for T4, we made a microfluidics-based noncompetitive immunodetection chip system using anti-T4 antibody fragments obtained from a phage display library.
Results:
Based on the open-sandwich ELISA principle that detects antigen-dependency of the interaction between the two antibody variable regions V(H) and V(L), we could detect less than 1 ng/ml of T4. The assay was also successfully applied to evaluate total T4 concentration in the serum of healthy individuals.
Conclusion:
This would be the first micro open-sandwich ELISA constructed with antibody fragments directly selected from immunized mice. The system will be applied to the sensitive detection of many diagnostic markers.
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