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04:39
Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model
Published on: March 17, 2023
Micro open-sandwich ELISA to rapidly evaluate thyroid hormone concentration from serum samples
Kamrun Nahar Islam1, Masaki Ihara, Jinhua Dong
1Department of Chemistry & Biotechnology, University of Tokyo, Tokyo, Japan.
Bioanalysis
|November 19, 2010
Summary
This study developed a rapid and sensitive microfluidics assay for thyroxine (T4) detection. The novel system successfully measured T4 in human serum, paving the way for advanced diagnostic tools.
Area of Science:
- Biotechnology
- Immunotechnology
- Analytical Chemistry
Background:
- Thyroxine (T4) is a critical thyroid hormone for diagnosing thyroid function.
- Existing assays require improvement in speed and sensitivity for T4 measurement.
Purpose of the Study:
- To develop a rapid and sensitive microfluidics-based immunodetection chip system for T4.
- To utilize anti-T4 antibody fragments selected via phage display for assay development.
Main Methods:
- Development of a microfluidics chip system.
- Application of an open-sandwich ELISA principle using antibody variable regions (V(H) and V(L)).
- Selection of anti-T4 antibody fragments from a phage display library.
Main Results:
- Achieved detection of T4 at concentrations below 1 ng/ml.
- Demonstrated successful application of the assay for measuring total T4 in human serum.
- Established a micro open-sandwich ELISA using antibody fragments selected from immunized mice.
Conclusions:
- This represents the first micro open-sandwich ELISA utilizing antibody fragments directly selected from immunized mice.
- The developed system offers a sensitive method for T4 detection.
- The platform has potential for sensitive detection of various diagnostic markers.
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