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Related Experiment Videos

A paper membrane filter assay for ciliate chemoattraction.

V Leick1, K Frederiksen, I Lyhne

  • 1Department of Biochemistry B. Panum Institute, University of Copenhagen, Denmark.

Analytical Biochemistry
|January 1, 1990
PubMed
Summary

This study introduces a new quantitative bioassay to measure ciliate chemoattraction using Tetrahymena. The assay effectively detects responses to chemoattractants like proteose peptone and platelet-derived growth factor.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Microbiology

Background:

  • Chemoattraction is crucial for microbial behavior and survival.
  • Existing bioassays for ciliate chemoattraction can be complex or lack precision.
  • Tetrahymena species are well-established model organisms for cellular studies.

Purpose of the Study:

  • To develop and validate a quantitative bioassay for ciliate chemoattraction.
  • To assess the efficacy of the assay using Tetrahymena thermophila and Tetrahymena pyriformis.
  • To demonstrate the assay's utility with known chemoattractants.

Main Methods:

  • A modified Boyden chamber assay was employed, separating two compartments with a filter.
  • Starved Tetrahymena cells were introduced into one compartment, and chemoattractants into the other.

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  • Cell migration through the filter was quantified using electronic cell counting.

    • Main Results:

    • The developed bioassay provides a convenient and quantitative measure of ciliate chemoattraction.
    • A signal-to-noise ratio of approximately 10 was achieved, indicating assay reliability.
    • The assay successfully detected chemoattraction in response to proteose peptone and platelet-derived growth factor.

    Conclusions:

    • The described quantitative bioassay is a reliable method for studying ciliate chemoattraction.
    • This assay offers a practical tool for research in microbial chemotaxis and signaling.
    • The findings support the use of Tetrahymena as model organisms in chemoattraction studies.