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Related Concept Videos

Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

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Updated: Jun 6, 2026

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

Human telomerase activity regulation.

Aneta Wojtyla1, Marta Gladych, Blazej Rubis

  • 1Department of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, Przybyszewskiego 49 St, 60-355 Poznan, Poland.

Molecular Biology Reports
|November 19, 2010
PubMed
Summary
This summary is machine-generated.

Telomerase, crucial for cancer cell proliferation, can be targeted for therapy. Beyond gene expression, post-transcriptional regulation of telomerase offers novel anticancer strategies by controlling enzyme activity.

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Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
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Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

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Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
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Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay
10:14

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay

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Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
08:26

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Published on: June 12, 2018

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Telomerase is a key differentiator between normal and cancer cells, making it a significant therapeutic target.
  • Cancer cells overcome replicative senescence via telomerase activity or the alternative lengthening of telomeres (ALT) pathway.
  • Telomerase regulation extends beyond gene expression, involving post-translational modifications and complex assembly.

Purpose of the Study:

  • To review and highlight post-transcriptional mechanisms regulating telomerase activity.
  • To explore novel therapeutic targets for anticancer strategies.
  • To emphasize that targeting telomerase expression alone may be insufficient for effective cancer therapy.

Main Methods:

  • Literature review of post-transcriptional telomerase regulation.
  • Analysis of factors influencing telomerase assembly, transport, and phosphorylation.
  • Correlation of gene expression with enzyme activity.

Main Results:

  • Telomerase activity is regulated by multiple post-transcriptional mechanisms, including phosphorylation and subunit transport.
  • Gene expression levels do not always correlate with active telomerase enzyme levels.
  • Dysregulation of these post-transcriptional controls can be exploited for therapeutic intervention.

Conclusions:

  • Targeting telomerase expression alone may not be sufficient for successful cancer therapy.
  • Post-transcriptional regulation of telomerase presents promising new avenues for developing anticancer treatments.
  • Understanding these complex regulatory mechanisms is crucial for advancing telomere-based cancer therapies.