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Updated: Jun 6, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
Published on: July 12, 2022
The SNO-proteome: causation and classifications
Divya Seth1, Jonathan S Stamler
1Institute for Transformative Molecular Medicine, Case Western Reserve University School of Medicine and University Hospitals, Cleveland, OH 44106, USA.
Abstract:
Cell signaling is a complex and highly regulated process. Post-translational modifications of proteins serve to sense and transduce cellular signals in a precisely coordinated manner. It is increasingly recognized that protein S-nitrosylation, the addition of a nitric oxide group to cysteine thiols, serves an important role in a wide range of signaling pathways. In spite of the large number of SNO-proteins now identified (∼1000), the observed specificity of S-nitrosylation in terms of target proteins and specific cysteines within modified proteins is incompletely understood. Here we review the progress made in S-nitrosylation detection methods that have facilitated the study of the SNO-proteome under physiological and pathophysiological conditions, and some factors important in determining the SNO-proteome. Classification schemes for emergent denitrosylases and prospective 'protein S-nitrosylases' are provided.
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