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Localization of an origin of replication in Corynebacterium diphtheriae broad host range plasmid pNG2 that also
T M Serwold-Davis1, N B Groman, C C Kao
1Department of Microbiology, School of Medicine, University of Washington, Seattle 98195.
FEMS Microbiology Letters
|January 1, 1990
Abstract:
Subcloning and protoplast transformation studies identified a 2.6 kb fragment of Corynebacterium diphtheriae plasmid pNG2 which contains an origin of replication (oriR). Molecular combination of the 2.6 kb oriR cartridge with Escherichia coli plasmid pUC18CmR enabled the E. coli cloning vector to replicate within several species of Corynebacterium host cells. A 2.6 kb plasmid formed from the oriR cartridge alone is capable of replicating in E. coli. This suggests that a single origin could be used in vectors shuttling between Corynebacterium spp. and E. coli.