Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...

Structure and Function of Platelets

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...

Formation of the Platelet Plug

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...

Pathophysiology of Cardiac Performance

Pathophysiology of Cardiac Performance

Typical heart performance is influenced by heart rate, rhythm, myocardial contraction, and metabolism or blood flow. The cardiac muscle exhibits distinct electrophysiological features, including pacemaker activity and calcium channel control, which play a vital role in the heart's response to various drugs. The autonomic nervous system, comprising the sympathetic and parasympathetic branches, regulates heart rate. Sympathetic activation increases heart rate, while parasympathetic activation...

Time Course of Drug Effect

Time Course of Drug Effect

The progression of a drug's impact can be analyzed by examining both the concentration-time course and the effect-time course. The concentration-time course is determined by the drug's half-life and is influenced by factors such as its pharmacokinetics, including absorption, distribution, metabolism, and elimination. The effect of the drug is often related to its concentration in the plasma and is calculated using the maximum drug effect and the plasma concentration that generates 50 percent of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Targeting Circular RNAs (circRNAs) in Atherosclerosis Using CRISPR Technology.

The journal of gene medicine·2025

Same author

Protective effect of <i>Pterospermum rubiginosum</i> bark extract on bone mineral density and bone remodelling in estrogen deficient ovariectomized Sprague-Dawley (SD) rats.

3 Biotech·2024

Same author

Alcohol induced NLRP3 inflammasome activation in the brain of rats is attenuated by ATRA supplementation.

Brain, behavior, & immunity - health·2024

Same author

Targeting the Role of PRME in Regulating Bone Remodelling During Postmenopausal Osteoporosis.

Chemistry & biodiversity·2024

Same author

An integrated approach to the structural characterization, long-term toxicological and anti-inflammatory evaluation of Pterospermum rubiginosum bark extract.

Journal of ethnopharmacology·2023

Same author

Nanohydroxyapatite incorporated photocrosslinked gelatin methacryloyl/poly(ethylene glycol)diacrylate hydrogel for bone tissue engineering.

Progress in biomaterials·2021

Same journal

Dr. A.P.J. Abdul Kalam : A Humble Tribute.

Indian journal of physiology and pharmacology·2024

Same journal

Assessment of EEG as a Diagnostic and Prognostic Indicator Tool in the Febrile Seizures.

Indian journal of physiology and pharmacology·2024

Same journal

Ictal EEG Non-linear And High Order Spectral Analysis Methods In Electroconvulsive Therapy And Its Clinical Utility.

Indian journal of physiology and pharmacology·2024

Same journal

Nitric Oxide Synthases Blockade; L-NAME and Estradiol Alter Renal Blood Flow Response to Angiotensin 1-7 in Ovariectomized Rats.

Indian journal of physiology and pharmacology·2024

Same journal

Leptin, Adiponectin and its Molar Ratio as a Biomarker in the Diagnosis of Metabolic Syndrome.

Indian journal of physiology and pharmacology·2024

Same journal

The Effect of Maternal Age, Parity and Heamoglobin Level on Neonatal Birth Weight - A Fru Based Prevalence Study.

Indian journal of physiology and pharmacology·2024

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 6, 2026

Microfluidics in Assessing Platelet Function

Microfluidics in Assessing Platelet Function

Published on: November 8, 2024

Modulatory effect of coffee on platelet function.

Shobha Bhaskar¹, Arun A Rauf

¹Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram.

Indian Journal of Physiology and Pharmacology

|November 25, 2010

Summary

This summary is machine-generated.

Moderate coffee consumption may inhibit platelet aggregation, a key factor in cardiovascular disease and thrombosis. This study found coffee reduced platelet activation markers in rats, suggesting a potential cardioprotective effect.

More Related Videos

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor

Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor

Published on: May 2, 2025

Related Experiment Videos

Last Updated: Jun 6, 2026

Microfluidics in Assessing Platelet Function

Microfluidics in Assessing Platelet Function

Published on: November 8, 2024

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor

Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor

Published on: May 2, 2025

Area of Science:

Cardiovascular Science
Hematology
Nutritional Science

Background:

Blood platelets are crucial in cardiovascular disease (CVD) and thrombosis.
The impact of coffee consumption on cardiovascular health remains debated.
Platelet function and primary hemostasis are key areas of investigation.

Purpose of the Study:

To investigate the effects of moderate coffee consumption on platelet function and primary hemostasis.
To assess these effects in both normal and high-fat diet-fed rats.
To determine coffee's role in modulating platelet aggregation in vivo.

Main Methods:

Comparative study in rats fed normal, high-fat, or high-fat plus coffee diets.
Analysis of platelet parameters: mean platelet volume, platelet crit, and platelet distribution width.
Assessment of platelet aggregation induced by various agonists (ADP, collagen, arachidonic acid, epinephrine).
Measurement of malondialdehyde concentration in platelets.
Scanning electron microscopy for platelet morphology and aggregation.

Main Results:

Coffee consumption significantly decreased mean platelet volume, platelet crit, and platelet distribution width compared to the high-fat diet group.
Malondialdehyde concentration, an indicator of thromboxane A2 production, was reduced in the coffee-treated group.
Platelet aggregation induced by ADP, collagen, arachidonic acid, and epinephrine was significantly inhibited in the coffee-fed group.
Scanning electron microscopy confirmed reduced platelet aggregation tendency in rats consuming coffee.

Conclusions:

Moderate coffee consumption demonstrates inhibitory effects on platelet aggregation.
Coffee consumption may play a role in reducing the risk of thrombosis.
These findings suggest a potential cardioprotective mechanism for coffee through modulation of platelet activity.