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Published on: May 5, 2017
Induction of BAP1 by a moderate decrease in temperature is mediated by ICE1 in Arabidopsis
Ying Zhu1, Huijun Yang, Hyung-Gon Mang
1Department of Plant Biology, Cornell University, Ithaca, New York 14853, USA.
Plant Physiology
|November 25, 2010
Summary
Plants perceive moderate cooling through the BON1-associated protein1 (BAP1) gene, involving the ICE1 protein. This discovery sheds light on temperature signaling pathways and potential pathogen resistance mechanisms in plants.
Area of Science:
- Plant Physiology
- Molecular Biology
- Gene Regulation
Background:
- Nonextreme temperature variations significantly impact plant growth and physiology.
- Understanding plant perception and signal transduction of temperature changes is crucial but remains limited.
Purpose of the Study:
- To investigate the transcriptional control of the BON1-associated protein1 (BAP1) gene.
- To identify the specific DNA regulatory elements and proteins involved in BAP1 gene induction by moderate cooling.
Main Methods:
- Reporter gene assays were used to analyze the BAP1 promoter activity.
- Electrophoretic mobility shift assays (EMSA) or similar techniques were implied to study protein-DNA interactions.
- Analysis of the ice1 mutant to determine the role of ICE1 in BAP1 regulation.
Main Results:
- A 35-bp promoter fragment of BAP1 was identified as essential and sufficient for induction by moderate cooling.
- This fragment also mediated induction by cold stress and paraquat (ROS inducer).
- The inducer of CBF expression1 (ICE1) protein binds to a MYC element in the BAP1 promoter and is required for cooling-induced BAP1 expression.
Conclusions:
- Moderate cooling induces BAP1 transcription via a specific promoter fragment.
- The ICE1 protein plays a critical role in mediating BAP1 induction by moderate temperature decrease.
- Plant responses to moderate cooling may involve components of the cold response pathway and salicylic acid signaling.
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