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Related Concept Videos

Matrix Proteoglycans and Glycoproteins01:21

Matrix Proteoglycans and Glycoproteins

Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...
The Extracellular Matrix01:42

The Extracellular Matrix

In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.Composition of the Extracellular MatrixThe extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules.
The Extracellular Matrix01:29

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Integrins01:10

Integrins

Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Extracellular Matrix01:26

Extracellular Matrix

Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...

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Related Experiment Video

Updated: Jun 6, 2026

Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles
08:04

Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles

Published on: November 3, 2023

Extracellular matrix proteins involved in pseudoislets formation.

Elisa Maillard1, Marie-Christine Sencier, A Langlois

  • 1Centre Européen d'Etude du Diabète, Strasbourg, France.

Islets
|November 25, 2010
PubMed
Summary

Extracellular matrix proteins, including laminin 5 and collagen IV, are crucial for forming pancreatic pseudoislets. These proteins enhance insulin expression and secretion, suggesting improved pancreatic islet function.

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Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles
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Published on: December 16, 2021

Area of Science:

  • Cell Biology
  • Biochemistry
  • Endocrinology

Background:

  • Extracellular matrix (ECM) proteins mediate cell adhesion via integrins.
  • ECM proteins influence cellular processes, including insulin expression and secretion in pancreatic islets.

Purpose of the Study:

  • To investigate the role of ECM proteins in the formation of pancreatic pseudoislets.
  • To analyze the expression of insulin, collagen IV, fibronectin, laminin 5, and β1-integrin during pseudoislet development.

Main Methods:

  • Culture of the β-cell line RINm5F to form adherent cells or pseudoislets.
  • Analysis of gene and protein expression using RT-PCR and immunohistochemistry.
  • Time-course study over 1, 3, 5, and 7 days of culture.

Main Results:

  • Insulin expression and secretion increased during pseudoislet formation.
  • Collagen IV expression was transient in pseudoislets (first 3 days), while laminin 5 expression was higher in pseudoislets than adherent cells.
  • β1-integrin expression increased in pseudoislets, and fibronectin expression was higher in adherent cells.

Conclusions:

  • Laminin 5 and collagen IV are implicated in pseudoislet formation.
  • Fibronectin appears involved in cell adhesion, while ECM proteins may enhance pseudoislet function.
  • Pseudoislet formation involves dynamic changes in ECM protein expression and cellular organization.