Related Experiment Videos
Purification of soluble enzymes from erythrocyte hemolysates by three phase partitioning
M C Pol1, H F Deutsch, L Visser
1Department of Biochemistry, University of Pretoria, South Africa.
The International Journal of Biochemistry
|January 1, 1990
Abstract:
1. The three phase partitioning method of protein fractionation was successfully applied to human erythrocyte hemolysates for the removal of hemoglobin and the concentration of soluble enzymes. 2. Human carbonic anhydrase I and II, catalase and superoxide dismutase were recovered free of hemoglobin and in good yield in the initial partitioning step, with a 60- to 80-fold enrichment of enzyme activities. 3. After further purification, carbonic anhydrases I and II were obtained at overall yields of 84 and 29%, respectively, crystallized catalase at 38% and superoxide dismutase at 52%.