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Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining
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Published on: December 23, 2016

Possible role of infectious simian foamy virus DNA in persistent infection

C T Chu, J J Hooks

    Zhonghua Minguo Wei Sheng Wu Xue Za Zhi = Chinese Journal of Microbiology
    |December 1, 1977
    PubMed

    Insights

    Simian foamy virus type 7 (SFV-7) persists in rabbit leukocytes. Infectious SFV-7 DNA in kidney cells suggests a mechanism for viral persistence during replication.

    Area of Science:

    • Virology
    • Immunology
    • Molecular Biology

    Background:

    • Simian foamy viruses (SFVs) are retroviruses known for establishing persistent infections in various hosts.
    • Understanding the persistence mechanisms of SFV-7 is crucial for studying retroviral latency and host-virus interactions.

    Purpose of the Study:

    • To investigate the persistence of Simian foamy virus type 7 (SFV-7) in rabbit peripheral blood leukocytes.
    • To explore potential mechanisms contributing to SFV-7 persistence in infected rabbits.

    Main Methods:

    • In vitro culture of rabbit peripheral blood leukocytes from SFV-7 infected rabbits.
    • Detection of SFV-7 in cultured leukocytes.
    • Infectivity assays using DNA from SFV-7 infected rabbit kidney cells.

    Main Results:

    • SFV-7 was found to persist in peripheral blood leukocytes of infected rabbits.
    • Rabbit leukocytes cultured in vitro did not support SFV-7 replication but allowed low-titer virus detection after 4-5 days.
    • DNA derived from infected rabbit kidney cells demonstrated infectivity, suggesting the presence of infectious provirus.

    Conclusions:

    • SFV-7 can persist in rabbit peripheral blood leukocytes.
    • The presence of infectious SFV-7 DNA provirus in infected kidney cells likely contributes to viral persistence during replication.

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