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Updated: Jun 6, 2026
07:12
A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing
Published on: July 28, 2023
Efficient and scalable serial extraction of DNA and RNA from frozen tissue samples
Lucy Mathot1, Monica Lindman, Tobias Sjöblom
1Department of Genetics and Pathology, Uppsala University, SE-751 85 Uppsala, Sweden.
Chemical Communications (Cambridge, England)
|November 25, 2010
Abstract:
Advances in cancer genomics have created a demand for scalable sample processing. We here present a process for serial extraction of nucleic acids from the same frozen tissue sample based on magnetic silica particles. The process is automation friendly with high recoveries of pure DNA and RNA suitable for analysis.
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