Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Agnostic material classification using differential de Bruijn graphs of DNA imprints.

bioRxiv : the preprint server for biology·2026
Same author

Identifying membrane-bound transcriptional regulatory proteins from rare but evolutionarily conserved domain combinations.

Nucleic acids research·2026
Same author

A cross-vertebrate brain protein interaction map identifies conserved neural and non-neural complexes.

Cell reports·2026
Same author

A protein interactome for the last eukaryotic common ancestor illuminates the biochemical basis of modern genetic diseases.

Cell genomics·2026
Same author

Comparative proteomic profiling of receptor kinase signaling reveals key trafficking components enforcing plant stomatal development.

Science advances·2026
Same author

Validation and analysis of 12,000 AI-driven CAR-T designs in the <i>Bits to Binders</i> competitions.

bioRxiv : the preprint server for biology·2026
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jun 6, 2026

A high-throughput method to globally study the organelle morphology in S. cerevisiae
07:29

A high-throughput method to globally study the organelle morphology in S. cerevisiae

Published on: March 2, 2009

High-throughput immunofluorescence microscopy using yeast spheroplast cell-based microarrays.

Wei Niu1, G Traver Hart, Edward M Marcotte

  • 1Department of Genetics, Yale University, New Haven, CT, USA. wei.niu08@gmail.com

Methods in Molecular Biology (Clifton, N.J.)
|November 25, 2010
PubMed
Summary
This summary is machine-generated.

We developed a high-throughput method using yeast cell microarrays for microscopy. This technique enables efficient genome-wide screening of single-cell phenotypes and genetic assays.

More Related Videos

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast
06:52

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast

Published on: January 23, 2012

Fission Yeast as a Platform for Antibacterial Drug Screens Targeting Bacterial Cytoskeleton Proteins
05:57

Fission Yeast as a Platform for Antibacterial Drug Screens Targeting Bacterial Cytoskeleton Proteins

Published on: April 26, 2024

Related Experiment Videos

Last Updated: Jun 6, 2026

A high-throughput method to globally study the organelle morphology in S. cerevisiae
07:29

A high-throughput method to globally study the organelle morphology in S. cerevisiae

Published on: March 2, 2009

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast
06:52

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast

Published on: January 23, 2012

Fission Yeast as a Platform for Antibacterial Drug Screens Targeting Bacterial Cytoskeleton Proteins
05:57

Fission Yeast as a Platform for Antibacterial Drug Screens Targeting Bacterial Cytoskeleton Proteins

Published on: April 26, 2024

Area of Science:

  • Cell biology
  • Microscopy techniques
  • Yeast genetics

Background:

  • High-throughput screening is crucial for understanding complex biological systems.
  • Yeast cell-based assays offer a powerful model for genetic and phenotypic studies.
  • Current methods for analyzing large yeast collections can be labor-intensive.

Purpose of the Study:

  • To establish a novel protocol for high-throughput immunofluorescence microscopy on yeast cell microarrays.
  • To enable genome-wide screening of yeast strains for single-cell phenotypes.
  • To facilitate systematic localization and genetic assays in yeast.

Main Methods:

  • Printing spheroplasted yeast cells onto high-density microarrays (over 5,000 spots per array).
  • Performing immunostaining on the yeast spheroplast microarrays.
  • Utilizing automated microscopy for high-throughput imaging and analysis.

Main Results:

  • Demonstrated the feasibility of generating yeast spheroplast microarrays for large-scale analysis.
  • Successfully applied the method to probe microtubule and spindle defects in essential yeast genes.
  • Showcased the utility for systematic localization and genetic assays.

Conclusions:

  • Yeast spheroplast microarrays provide an efficient platform for high-throughput microscopy.
  • This protocol is suitable for genome-wide screens of single-cell phenotypes.
  • The method facilitates the study of gene function and cellular processes in yeast.