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Published on: June 8, 2022
[Research on the blood components detecting by multi-optical path length spectroscopy technique]
1State Key Laboratory of Precision Measurement Technology and Instruments, Tianjin University, Tianjin 300072, China. ligang59@tju.edu.cn
Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|November 26, 2010
Summary
This study demonstrates the feasibility of using multi-optical path length spectroscopy to measure key human blood components like glucose and cholesterol. The developed technique offers a convenient and rapid method for blood analysis.
Area of Science:
- Analytical Chemistry
- Biomedical Engineering
- Spectroscopy
Background:
- Accurate measurement of human blood components is crucial for disease diagnosis and monitoring.
- Existing methods for blood analysis can be time-consuming and require specialized laboratory equipment.
- Developing rapid, non-invasive, or minimally invasive techniques for blood component analysis is a significant area of research.
Purpose of the Study:
- To evaluate the feasibility of using serum multi-optical path length spectroscopy for quantifying human blood components.
- To develop a quantitative analysis model for glucose (GLU), total cholesterol (TC), total protein (TP), and albumin (ALB) in serum.
- To establish a foundation for convenient and rapid detection of blood and other liquid components.
Main Methods:
- An automatic micro-displacement measuring device was designed to obtain near-infrared multi-optical path length spectra (0-4.0 mm) from 200 serum samples.
- Partial Least Squares (PLS) regression was employed to build quantitative analysis models using spectral data from 160 samples.
- The predictive accuracy of the models was validated on an independent set of 40 serum samples.
Main Results:
- The developed quantitative analysis models demonstrated favorable prediction effects for glucose, total cholesterol, total protein, and albumin.
- Correlation coefficients (r) between predicted and biochemical analysis values were high, ranging from 0.9320 to 0.9712.
- The study successfully proved the feasibility of the multi-optical path length spectroscopy technique for analyzing blood components.
Conclusions:
- Multi-optical path length spectroscopy is a feasible technique for the quantitative analysis of human blood components in serum.
- This spectroscopic approach provides a foundation for developing convenient and rapid methods for detecting blood and other liquid analytes.
- The findings support the potential of optical spectroscopy for point-of-care diagnostics and routine health monitoring.
