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Updated: Jun 6, 2026

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
Published on: September 16, 2014
[Study on method of time-resolved fluorescence spectra for rare fluorescence chelate]
Yun-hui Li1, Yan-qing Di, Xiu-yun Yang
1College of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun 130022, China. liyh@cust.edu.cn
Abstract:
In the present paper, the technology of time-resolved fluorescence spectra was investigated in order to improve the sensitivity of the solid-phase time-resolved fluorescence immunoassay. The fluorescence properties, fluorescence intensity and detection method of the novel complexes 4,4'-dibromo-6,6'-bis(N,N-bis(ethoxycarbonylmethyl) amino methyl)-2,2'-bipyridine were investigated through self-developed solid-phase time-resolved fluorescence immunoassay instrument. At the same time, the Eu-TTA-phen and Eu-beta-NTA-TOPO series of Eu(III) compounds were detected.
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