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A Murine Model of Group B Streptococcus Vaginal Colonization
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Published on: November 16, 2016

Multilocus sequence types in Czech neonatal GBS strains from 2004 to 2008.

L Straková1, M Musílek, J Motlová

  • 1National Institute of Public Health, Prague. lenkastrakova@yahoo.com

Epidemiologie, Mikrobiologie, Imunologie : Casopis Spolecnosti Pro Epidemiologii a Mikrobiologii Ceske Lekarske Spolecnosti J.E. Purkyne
|November 26, 2010
PubMed
Summary

The first multilocus sequence typing of Czech neonatal Streptococcus agalactiae reveals 3 main sequence types (ST17, ST23, ST19) dominate invasive isolates. Hyperinvasive ST17 is strongly linked to serotype III and cerebrospinal fluid infections.

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Last Updated: Jun 6, 2026

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10:19

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Published on: November 16, 2016

Area of Science:

  • Microbiology
  • Genetics
  • Neonatal Health

Background:

  • Streptococcus agalactiae (Group B Streptococcus) is a leading cause of neonatal infections.
  • Understanding the genetic diversity and invasive potential of S. agalactiae is crucial for effective prevention and treatment strategies.
  • Multilocus sequence typing (MLST) is a powerful tool for characterizing bacterial population structures.

Purpose of the Study:

  • To perform the first MLST analysis of Czech neonatal S. agalactiae isolates.
  • To determine if Czech isolates belong to the invasive clonal complex CC17.
  • To investigate the relationship between serotypes and sequence types (STs) in invasive isolates.

Main Methods:

  • Multilocus sequence typing (MLST) was performed on 77 neonatal S. agalactiae isolates from the Czech Republic.
  • Sequence types were assigned to clonal complexes (CCs).
  • Serotype-sequence type associations were analyzed.

Main Results:

  • Twelve sequence types were identified, assigned to 5 clonal complexes.
  • A high degree of sequence type variability was observed for most serotypes.
  • Three sequence types (ST17, ST23, ST19) accounted for 83% of invasive isolates.
  • ST17 was exclusively associated with serotype III.
  • The 'hyperinvasive' ST17 was found in 85% of cerebrospinal fluid isolates and 32% of blood isolates.

Conclusions:

  • Czech neonatal S. agalactiae isolates exhibit genetic diversity, with specific sequence types dominating invasive disease.
  • The clonal complex CC17, particularly ST17, is a significant factor in invasive neonatal infections, especially those involving serotype III and central nervous system disease.
  • MLST provides valuable insights into the population structure and invasive potential of S. agalactiae in neonates.