Apoptotic effect of alpha-mangostin on head and neck squamous carcinoma cells
Ruchadaporn Kaomongkolgit1, Niratcha Chaisomboon, Prasit Pavasant
1Department of Oral Diagnosis, Faculty of Dentistry, Naresuan University, Mueng, Phitsanulok, Thailand. ruchadapornk@yahoo.com
Objective:
The purposes of this study were to measure the cytotoxic effect of alpha-mangostin on head and neck squamous cell carcinoma (HNSCC) cell lines, to evaluate the apoptotic aspect of dead cells, and to identify the molecular mechanism involved in apoptosis.
Methods:
The human HNSCC cell lines HN-22, HN-30 and HN-31 were treated with alpha-mangostin. The apoptotic effects of alpha-mangostin on HNSCC cells were determined by observation the morphological changes of cells, immunofluorescence for single-stranded DNA (ssDNA), and DNA fragmentation analysis. The expression of bax, bcl-2, and p53 were detected by RT-PCR and Western blot analysis.
Results:
Alpha-mangostin showed excellent apoptotic effects on HNSCC cell lines, which induced the down-regulation of bcl-2, but up-regulation of bax and p53 in HN-22, HN-30 and HN-31.
Conclusion:
The present study suggests that the induction of apoptosis by alpha-mangostin seemed to be modulated by bcl-2, bax and p53 level in HNSCC cell lines.
Insights
Alpha-mangostin effectively induces apoptosis in head and neck squamous cell carcinoma (HNSCC) cell lines by altering key protein expressions. This natural compound shows promise for HNSCC treatment by modulating apoptotic pathways.
Area of Science:
- Oncology
- Molecular Biology
- Pharmacology
Background:
- Head and neck squamous cell carcinoma (HNSCC) is a significant global health concern.
- Developing novel therapeutic agents for HNSCC remains a critical research area.
- Alpha-mangostin, a natural xanthone, has demonstrated potential anti-cancer properties.
Purpose of the Study:
- To investigate the cytotoxic effects of alpha-mangostin on HNSCC cell lines.
- To analyze the apoptotic mechanisms induced by alpha-mangostin.
- To identify the molecular targets involved in alpha-mangostin-mediated apoptosis.
Main Methods:
- Treatment of HNSCC cell lines (HN-22, HN-30, HN-31) with alpha-mangostin.
- Assessment of apoptosis via morphological changes, ssDNA immunofluorescence, and DNA fragmentation.
- Analysis of gene and protein expression levels of bax, bcl-2, and p53 using RT-PCR and Western blot.
Main Results:
- Alpha-mangostin exhibited significant apoptotic effects on HNSCC cell lines.
- The compound induced down-regulation of bcl-2 expression.
- Alpha-mangostin led to the up-regulation of bax and p53 expression.
Conclusions:
- Alpha-mangostin effectively induces apoptosis in HNSCC cells.
- The apoptotic process appears to be modulated by the levels of bcl-2, bax, and p53.
- Alpha-mangostin represents a potential therapeutic candidate for HNSCC.