Rapid and sensitive loop-mediated isothermal amplification test for Clostridium difficile detection challenges
Torbjörn Norén1, Ingegärd Alriksson, Josefin Andersson
1Department of Laboratory Medicine, Microbiology, Örebro University Hospital, SE-701 85 Örebro, Sweden. torbjorn.noren@orebroll.se
Abstract:
Compared to the composite gold standard cytotoxin B assay and toxigenic culture, the loop-mediated isothermal amplification (LAMP) test for Clostridium difficile had a sensitivity and specificity of 98%, positive predictive value of 92%, and negative predictive value of >99%. A one-hour turnaround time for the LAMP test provides rapid diagnosis and cost savings.
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