The 2-dot technique: minimalist donor lenticule marking in endothelial keratoplasty

Correct orientation of the donor lenticule within the anterior chamber is essential for the success of Descemet stripping automated endothelial keratoplasty. We describe a 2-dot pattern for gentian violet marking of the donor lenticule that confirms correct graft positioning while minimizing associated endothelial toxicity. The 2-dot pattern is the smallest pattern possible with no axis of rotational symmetry about any diameter across the lenticule.