Related Experiment Video
Updated: Jun 6, 2026
11:12
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
RAS polymorphisms in cancerous and benign breast tissue
Adriana P Mendizábal-Ruiz1, José Morales, Xochitl Castro Martinez
1Universidad de Guadalajara, Mexico.
Journal of the Renin-Angiotensin-Aldosterone System : JRAAS
|November 27, 2010
Summary
The renin-angiotensin system (RAS) genes are linked to breast cancer and benign breast disease (BBD). Specific gene polymorphisms, including ACE and AGTR1, show associations with increased or reduced risk in these conditions.
Area of Science:
- Genetics and Molecular Biology
- Oncology
- Cardiovascular Research
Background:
- The renin-angiotensin system (RAS) plays a role in angiogenesis.
- Limited research exists on the association between RAS genes and cancer, with a focus on ACE.
- Few studies have investigated specific RAS gene polymorphisms in breast cancer, particularly for REN, AGT, and AGTR1.
Purpose of the Study:
- To investigate the association of polymorphisms in REN, AGT, ACE, and AGTR1 genes with breast cancer.
- To explore the potential role of these RAS gene polymorphisms in benign breast disease (BBD).
Main Methods:
- Polymorphisms in REN (Bgll), AGT (M235T), ACE (A245T, Indel), and AGTR1 (A1166C) were analyzed.
- Polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFPLs) or sequence-specific assays were employed.
- Three groups were studied: breast cancer patients, patients with benign breast disease (BBD), and the general population.
Main Results:
- REN polymorphism (A allele homozygotes) was associated with an increased risk of BBD.
- AGT (M235T) genotype frequencies differed, with fewer heterozygotes in the breast cancer group.
- ACE indel polymorphism was associated with both BBD and breast cancer.
- AGTR1 (A1166C) polymorphism showed an association in the breast cancer group, with C allele carriers having a reduced risk.
Conclusions:
- The study supports the involvement of the renin-angiotensin system in breast cancer.
- RAS gene polymorphisms may also play a role in the development of benign breast disease.
- Further research is warranted to elucidate the complex role of RAS in breast pathologies.
Related Concept Videos
01:11Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase
Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
01:20The Retinoblastoma Gene
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
02:38The Ras Gene
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...
Ras is a superfamily...
01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
01:03Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
01:03Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...