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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

lncRNAdb: a reference database for long noncoding RNAs.

Paulo P Amaral1, Michael B Clark, Dennis K Gascoigne

  • 1Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia.

Nucleic Acids Research
|November 30, 2010
PubMed
Summary
This summary is machine-generated.

A new database, lncRNAdb, catalogs functional long noncoding RNAs (lncRNAs) and regulatory messenger RNAs in eukaryotes. This resource systematically compiles and updates information on RNA sequences, expression, function, and genomic context.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Eukaryotes possess numerous long noncoding RNAs (lncRNAs) with limited protein-coding potential.
  • Growing data exists on lncRNA expression, features, and functions across various biological systems.

Purpose of the Study:

  • To develop a comprehensive database (lncRNAdb) for systematic compilation and updating of lncRNA information.
  • To include messenger RNAs with known regulatory roles.

Main Methods:

  • Developed lncRNAdb, a curated database of functional lncRNAs and regulatory mRNAs.
  • Integrated data on RNA sequences, structure, genomic context, expression, localization, conservation, and functional evidence.
  • Enabled searching by RNA names, sequences, species, associated genes, expression tissues, and diseases.

Main Results:

  • lncRNAdb provides a centralized repository for lncRNA data.
  • The database links to external resources like the UCSC Genome Browser and NRED for enhanced visualization and expression analysis.
  • lncRNAdb facilitates ongoing literature collation on lncRNAs and their genomic associations.

Conclusions:

  • lncRNAdb serves as a valuable platform for researchers studying lncRNAs and their biological roles.
  • The database promotes systematic data management and accessibility in the field of noncoding RNA research.
  • lncRNAdb supports the exploration of lncRNA functions and their association with other genomic elements.