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Updated: Jun 6, 2026

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
Published on: September 28, 2017
Melatonin modifies the rhythm of protein synthesis
V Y Brodsky1, N D Dubovaya, T K Zvezdina
1N. K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia. brodsky.idb@bk.ru
Abstract:
Melatonin (5 nM) added to medium with primary hepatocyte cultures shifted the phase of circahoralian rhythm of protein synthesis and hence, can be a factor synchronizing fluctuations in protein synthesis and rhythm organizer in the hepatocyte population. Blockade of melatonin receptors with luzindole (20 nM) arrested rhythm organization of protein synthesis by melatonin. Prospects of studying biochemical mechanisms of protein synthesis rhythm organization with other drugs (calcium agonists, similarly to melatonin) are discussed.
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