Related Experiment Video
Updated: Jun 6, 2026
07:29
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Interrelation between malonyl dialdehyde-dependent modification and cholesterol content in low-density lipoproteins
A K Tikhaze1, M Viigimaa, G G Konovalova
1Russian Cardiology Research-and-Production Complex, Russian Medical Technologies, Moscow, Russia.
Bulletin of Experimental Biology and Medicine
|November 30, 2010
Summary
Oxidized LDL shows a strong correlation with atherosclerosis biomarkers in plasma. However, this link is weaker in diabetes patients, suggesting other LDL-modifying agents may be present.
Area of Science:
- Biochemistry
- Cardiovascular Medicine
- Metabolic Disorders
Background:
- Atherosclerosis is a major cause of cardiovascular disease.
- Lipid peroxidation, particularly of low-density lipoprotein (LDL), plays a key role in atherogenesis.
- Malondialdehyde (MDA)-modified LDL is a recognized marker of oxidative stress and a potential contributor to atherosclerosis.
Purpose of the Study:
- To investigate the correlation between MDA-modified LDL and established atherosclerosis biomarkers in a general population sample.
- To examine how this correlation differs in individuals with and without diabetes mellitus.
- To identify potential alternative LDL-modifying agents in diabetic patients.
Main Methods:
- Epidemiological study utilizing an independent, representative population sample.
- Analysis of blood plasma from 348 probands.
- Measurement of oxidized (MDA-modified) LDL levels.
- Quantification of atherosclerosis biomarkers: total cholesterol and LDL cholesterol.
Main Results:
- A strong positive correlation was observed between MDA-modified LDL and total/LDL cholesterol levels in the overall cohort.
- This correlation was more significant in patients diagnosed with atherosclerosis.
- The correlation was less pronounced in individuals with diabetes mellitus, and absent when considering atherosclerosis markers and MDA levels specifically.
- These findings suggest the presence of non-MDA LDL-modifying agents, such as glyoxal and methylglyoxal, in diabetic patients.
Conclusions:
- MDA-modified LDL is a significant biomarker for atherosclerosis in the general population.
- The diminished correlation in diabetes mellitus patients indicates a complex interplay of factors, including other potential LDL-modifying agents.
- MDA-modified LDL can serve as an additional, valuable biomarker for assessing atherosclerosis risk and progression.
Related Concept Videos
01:29Cholesterol: Significance and Regulation
Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
Considering cholesterol and...
01:28Overview of Fatty Acid Metabolism
Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
01:25Blood Studies for Cardiovascular System III: Serum Lipid Profile
Understanding serum lipids is crucial for maintaining cardiovascular health and preventing heart disease and stroke.
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
01:23Membrane Fluidity
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.Fatty acids tails of phospholipids can be either saturated or...
01:26Membrane Fluidity
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...
01:20Lipid-Lowering Drugs: Statins and Miscellaneous Agents
Hyperlipidemia, a medical condition often referred to as high cholesterol, is characterized by abnormally elevated levels of lipids in the bloodstream. When present in excess, these lipids, specifically cholesterol and triglycerides, can lead to serious health complications, often involving cardiovascular diseases. Illnesses like atherosclerosis, heart attacks, and pancreatitis have all been linked to untreated hyperlipidemia. This means controlling and regulating cholesterol and triglyceride...