Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Basic Science and Pathogenesis.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025

Same author

A model for predicting bacteremia species based on host immune response.

Frontiers in cellular and infection microbiology·2025

Same author

Integration of virtual and physical screening.

Drug discovery today. Technologies·2024

Same author

Best practices for managing and disseminating resources and outreach and evaluating the impact of the IDG Consortium.

Drug discovery today·2024

Same author

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs.

Journal of visualized experiments : JoVE·2024

Same author

Integrins as Therapeutic Targets for SARS-CoV-2.

Frontiers in cellular and infection microbiology·2022

Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 6, 2026

Compact Quantum Dots for Single-molecule Imaging

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Quantum dots for quantitative flow cytometry.

Tione Buranda¹, Yang Wu, Larry A Sklar

¹Department of Pathology and Cancer Center, University of New Mexico School of Medicine, Albuquerque, NM, USA. buranda@unm.edu

Methods in Molecular Biology (Clifton, N.J.)

|December 1, 2010

Summary

This summary is machine-generated.

Quantum dots offer a versatile alternative to traditional fluorophore calibration beads in flow cytometry. Their broad excitation and tunable emission enable precise quantitation of fluorescently labeled molecules across various spectral ranges.

More Related Videos

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

Published on: September 28, 2022

Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

Published on: June 17, 2020

Related Experiment Videos

Last Updated: Jun 6, 2026

Compact Quantum Dots for Single-molecule Imaging

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

Published on: September 28, 2022

Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

Published on: June 17, 2020

Area of Science:

Biotechnology
Analytical Chemistry
Spectroscopy

Background:

Flow cytometry relies on calibration beads for accurate quantitation of fluorophore-tagged molecules.
Existing fluorophore-based standards are limited by their specific excitation and emission spectra.
A need exists for broadly applicable and spectrally flexible calibration standards.

Purpose of the Study:

To introduce quantum dot (QD) fluorescence calibration beads as a superior alternative for flow cytometry.
To explore the spectroscopic principles and molecular assembly of QD-based calibration standards.
To demonstrate a generalizable method for calibrated fluorescence measurements using QDs.

Main Methods:

Investigated spectroscopic parameters (absorption coefficient ε, quantum yield φ, percent transmission %T) for fluorescence calibration.
Developed modular QD calibration beads with defined spectroscopic properties.
Focused on matching QD emission spectra to target fluorophores for accurate quantitation.

Main Results:

Quantum dots can be photo-excited over a broad spectral range, allowing for tunable emission.
QD calibration beads can be designed to match the spectral characteristics of various fluorophores.
Demonstrated the potential for precise site coverage quantitation using QD standards.

Conclusions:

Quantum dots provide a spectrally versatile platform for fluorescence calibration in flow cytometry.
QD-based beads overcome the limitations of traditional fluorophore standards.
This approach offers a generalizable method for accurate and flexible quantitative fluorescence measurements.