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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jun 6, 2026

Analysis of Cell Cycle Position in Mammalian Cells

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Multiparameter cell cycle analysis.

James W Jacobberger¹, R Michael Sramkoski, Tammy Stefan

¹Cytometry and Imaging Microscopy Core, Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH, USA.

Methods in Molecular Biology (Clifton, N.J.)

|December 1, 2010

Summary

This summary is machine-generated.

This study reviews cell cycle analysis methods, highlighting 5'-ethynyl-2'-deoxyuridine (EdU) as a novel S phase labeling technique equivalent to 5-bromo-2-deoxyuridine (BrdU) for cell biology research.

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Area of Science:

Cell biology
Cytometry
Molecular biology

Background:

Cell cycle analysis is crucial for understanding yeast, mammalian, and drosophila cell biology.
Traditional methods like DNA content analysis and 5-bromo-2-deoxyuridine (BrdU) incorporation are well-established.
Complex assays involve multiple antibody labeling for cell cycle-regulated proteins.

Purpose of the Study:

To present a concise methods section for mammalian cell cycle analysis.
To provide an extended notes section addressing problematic or poorly described aspects of cell cycle assays.
To introduce and evaluate 5 -ethynyl-2 -deoxyuridine (EdU) as a new S phase labeling method.

Main Methods:

DNA content analysis via cytometry.
Analysis of 5-bromo-2-deoxyuridine (BrdU) incorporation using antibodies.
Utilizing 5 -ethynyl-2 -deoxyuridine (EdU) incorporation for S phase labeling.
Multi-antibody labeling for cell cycle-regulated proteins.

Main Results:

EdU incorporation is presented as a new method for S phase labeling.
Published data suggests EdU incorporation is equivalent to BrdU incorporation.
The study focuses on practical challenges in multi-parameter cytometry for cell cycle analysis.

Conclusions:

EdU offers a viable alternative for S phase labeling in cell cycle studies.
Detailed methodological considerations are essential for complex cytometric assays.
This work aims to improve the reproducibility and understanding of cell cycle analysis techniques.