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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: Jun 6, 2026

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Published on: April 16, 2013

Standard practice for cell sorting in a BSL-3 facility.

Stephen P Perfetto¹, David R Ambrozak, Richard Nguyen

¹Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)

|December 1, 2010

Summary

This summary is machine-generated.

This guide details safe infectious cell sorting within a Biological Safety Level 3 (BSL-3) laboratory. It covers essential procedures and considerations for establishing and operating BSL-3 cell sorting facilities.

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Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

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Last Updated: Jun 6, 2026

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

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Published on: April 16, 2013

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

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Published on: August 7, 2021

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Published on: July 10, 2010

Area of Science:

Biosafety
Cell Sorting
Laboratory Management

Background:

Increasing numbers of Biological Safety Level 3 (BSL-3) and BSL-4 laboratories in the USA.
Growing demand for infectious cell sorting within BSL-3 environments.
Established ISAC Biosafety Committee standards for unfixed cell sorting.

Purpose of the Study:

To outline procedures for safe infectious cell sorting in a BSL-3 environment.
To facilitate the establishment and safe operation of BSL-3 cell sorting laboratories.
To address critical factors for BSL-3 laboratory setup.

Main Methods:

Risk assessment for cell sorter placement in BSL-3.
Implementation of containment verification protocols.
Adoption of remote operation and disinfection strategies.

Main Results:

Provides a framework for safe infectious cell sorting in BSL-3.
Highlights key operational considerations including PPE and instrument modifications.
Ensures enhanced aerosol evacuation for biosafety.

Conclusions:

Safe operation of infectious cell sorting in BSL-3 is achievable with proper planning and adherence to guidelines.
Comprehensive biosafety procedures are crucial for BSL-3 cell sorting.
This chapter serves as a vital resource for BSL-3 laboratory establishment and management.