Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Determination of Crystal Structures01:29

Determination of Crystal Structures

In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...
X-ray Crystallography02:18

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
Crystal Growth: Principles of Crystallization01:25

Crystal Growth: Principles of Crystallization

Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent – the...
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...
Crystal Field Theory - Octahedral Complexes02:58

Crystal Field Theory - Octahedral Complexes

Crystal Field Theory
To explain the observed behavior of transition metal complexes (such as colors), a model involving electrostatic interactions between the electrons from the ligands and the electrons in the unhybridized d orbitals of the central metal atom has been developed. This electrostatic model is crystal field theory (CFT). It helps to understand, interpret, and predict the colors, magnetic behavior, and some structures of coordination compounds of transition metals.
CFT focuses on...
Crystallographic Point Groups01:29

Crystallographic Point Groups

Crystallographic point groups represent the various symmetry operations that can occur within crystals. They are unique in that at least one point will always remain unchanged during these actions. For instance, consider the triclinic system. This system, devoid of any axis or plane of symmetry, aligns with the C1 and Ci point groups.where Cᵢ is characterized solely by a center of inversion.Contrastingly, the monoclinic system introduces an element of symmetry. This system with one plane and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Salinas-Giegé et al. tRNA Biology in Mitochondria. <i>Int. J. Mol. Sci.</i> 2015, <i>16</i>, 4518-4559.

International journal of molecular sciences·2026
Same author

Monomers, Dimers, and Oligomers of Pyroglutamate-Modified α-Synuclein Fragments Exhibit Distinct Biophysical Characteristics.

ACS chemical neuroscience·2025
Same author

Structure of the nucleosome-bound human BCL7A.

Nucleic acids research·2025
Same author

Protein crystallization and structure determination at room temperature in the CrystalChip.

FEBS open bio·2024
Same author

The tRNA identity landscape for aminoacylation and beyond.

Nucleic acids research·2023
Same author

Solution X-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes.

Protein science : a publication of the Protein Society·2023
Same journal

Effect of network topology on neuronal encoding based on spatiotemporal patterns of spikes.

HFSP journal·2010
Same journal

From genes to neural tube defects (NTDs): insights from multiscale computational modeling.

HFSP journal·2010
Same journal

Cytoskeletal dynamics in fission yeast: a review of models for polarization and division.

HFSP journal·2010
Same journal

Robustness versus evolvability: a paradigm revisited.

HFSP journal·2010
Same journal

Molecular motors as an auto-oscillator.

HFSP journal·2010
Same journal

Network reconstruction reveals new links between aging and calorie restriction in yeast.

HFSP journal·2010
See all related articles

Related Experiment Video

Updated: Jun 6, 2026

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
11:48

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

Biocrystallography: past, present, future.

Richard Giegé1, Claude Sauter

  • 1Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, 67084 Strasbourg, France.

HFSP Journal
|December 2, 2010
PubMed
Summary
This summary is machine-generated.

Biocrystallography has advanced significantly over 6 decades, overcoming challenges in sample preparation and structure determination. Future research will focus on complex structures, dynamic processes, and cellular integration for biomedical insights.

More Related Videos

Crystallization and In Situ Room Temperature Data Collection Using the Crystallization Facility at Harwell and Beamline VMXi, Diamond Light Source
07:08

Crystallization and In Situ Room Temperature Data Collection Using the Crystallization Facility at Harwell and Beamline VMXi, Diamond Light Source

Published on: March 8, 2024

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature
07:42

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature

Published on: March 11, 2022

Related Experiment Videos

Last Updated: Jun 6, 2026

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
11:48

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

Crystallization and In Situ Room Temperature Data Collection Using the Crystallization Facility at Harwell and Beamline VMXi, Diamond Light Source
07:08

Crystallization and In Situ Room Temperature Data Collection Using the Crystallization Facility at Harwell and Beamline VMXi, Diamond Light Source

Published on: March 8, 2024

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature
07:42

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature

Published on: March 11, 2022

Area of Science:

  • Structural Biology
  • Biophysics
  • Molecular Biology

Background:

  • Biocrystallography has evolved over six decades, driven by methodological and instrumental advancements.
  • The field's interdisciplinarity has fostered innovation by integrating physics and biology.
  • Key challenges in biocrystallography include sample preparation, crystallization, and structure elucidation.

Purpose of the Study:

  • To present the historical evolution of biocrystallography.
  • To discuss strategies for overcoming major bottlenecks in the field.
  • To explore future directions and frontiers in biocrystallography.

Main Methods:

  • Review of historical milestones and instrumental developments.
  • Analysis of strategies for macromolecular target selection, design, and characterization.
  • Discussion of crystallogenesis, crystal optimization, and 3D structure determination techniques.

Main Results:

  • Significant progress has been made in molecular sample preparation and structure elucidation.
  • Strategies to circumvent bottlenecks in target selection, crystallization, and analysis have been developed.
  • Biocrystallography has successfully determined structures of various molecular targets.

Conclusions:

  • Biocrystallography has matured into a global biology discipline.
  • Future frontiers include solving complex supramolecular assemblies and intrinsically unstructured proteins.
  • Developing "4D biology" and integrating structural data into cellular organization are key future goals.