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Southern Blot

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

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Related Experiment Video

Updated: Jun 6, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

Northern blotting analysis.

Knud Josefsen¹, Henrik Nielsen

¹The Bartholin Institute, Copenhagen University Hospital, Copenhagen, Denmark. knud@eln.dk

Methods in Molecular Biology (Clifton, N.J.)

|December 3, 2010

Summary

This summary is machine-generated.

Northern blotting is a reliable RNA analysis technique. Recent advancements enhance its sensitivity for detecting specific RNA levels and sizes in complex samples.

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Area of Science:

Molecular Biology
Genetics

Background:

Northern blotting is a standard technique for analyzing specific RNA.
It involves size fractionation, membrane transfer, and probe hybridization.

Purpose of the Study:

To describe the Northern blotting method.
To highlight its advantages and recent improvements.

Main Methods:

Gel electrophoresis for RNA size fractionation.
Blotting RNA onto a membrane.
Hybridization with labeled probes for detection.

Main Results:

Northern blotting accurately determines RNA size and steady-state levels.
The method is simple, inexpensive, and artifact-free.
Improved membranes and buffers increase sensitivity.

Conclusions:

Northern blotting remains a valuable tool for RNA analysis.
Enhanced sensitivity makes it competitive with other methods.