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Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
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ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Immunoprecipitation01:20

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Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

RIP-Chip analysis: RNA-Binding Protein Immunoprecipitation-Microarray (Chip) Profiling.

Ritu Jain1, Tiffany Devine, Ajish D George

  • 1Department of Biomedical Sciences, School of Public Health, Gen*NY*Sis Center for Excellence in Cancer Genomics, University at Albany-SUNY, Rensselaer, NY, USA. ritujain10@hotmail.com

Methods in Molecular Biology (Clifton, N.J.)
|December 3, 2010
PubMed
Summary
This summary is machine-generated.

mRNA binding proteins coordinate gene expression, similar to transcription factors. We pioneered RIP-Chip technology for global analysis of RNA-binding protein targets, advancing understanding of gene regulation networks.

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Chromatin Isolation by RNA Purification (ChIRP)
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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

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Chromatin Isolation by RNA Purification (ChIRP)
11:09

Chromatin Isolation by RNA Purification (ChIRP)

Published on: March 25, 2012

Area of Science:

  • Molecular Biology
  • Genomics
  • Gene Expression Regulation

Background:

  • Post-transcriptional regulation fine-tunes cellular processes via mRNA binding proteins.
  • These proteins coordinate translation of functionally related genes by binding mRNA subsets.
  • The networks governing this mRNA binding protein activity are not well understood.

Purpose of the Study:

  • To describe advances in the RNA-Binding Protein Immunoprecipitation-Microarray (RIP-Chip) technology.
  • To highlight the utility of RIP-Chip in globally analyzing mRNA subsets bound by RNA-binding proteins.
  • To further understand messenger ribonucleoprotein (mRNP) networks and gene expression regulation.

Main Methods:

  • Development and refinement of high-throughput genomic technologies.
  • Integration of genomic technologies with standard molecular biology tools.
  • Application of RNA-Binding Protein Immunoprecipitation-Microarray (RIP-Chip) Profiling, also known as ribonomic profiling.

Main Results:

  • Pioneered high-throughput RIP-Chip technology for global analysis of RNA-binding protein targets.
  • Revealed insights into the workings of mRNP networks within cells.
  • Advanced the understanding of gene expression regulation through post-transcriptional mechanisms.

Conclusions:

  • RIP-Chip is a powerful tool for studying mRNA binding protein networks.
  • This technology facilitates a global analysis of gene expression regulation.
  • Advances in RIP-Chip offer new avenues for dissecting complex cellular processes.