Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 6, 2026

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
10:46

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

Fluorescence-activated cell sorting for aptamer SELEX with cell mixtures.

Günter Mayer1, Marie-Sophie L Ahmed, Andreas Dolf

  • 1Department of Life and Medical Sciences, University of Bonn, Bonn, Germany.

Nature Protocols
|December 4, 2010
PubMed
Summary

This study presents a new method for selecting aptamers that specifically target desired cell subpopulations. This technique improves accuracy by reducing false positives, aiding in cell-specific therapeutics development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimising DNA origami assembly by reducing off-target interactions.

Nature communications·2026
Same author

Click Capture SELEX for the Identification of Click-Modified Aptamers Targeting Small Molecules in Solution.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Integrative omics and phase IIa clinical trial identify TNF as key node in autoimmune hepatitis.

Journal of hepatology·2026
Same author

High-throughput chemical proteomics workflow for profiling protein citrullination dynamics.

Nature communications·2026
Same author

Multimodal profiling of pancreatic cancer reveals a TIMP-1-dominated secretory profile determining pro-tumor immunoinstruction in human cancers.

Cell reports. Medicine·2026
Same author

Selection and Characterization of SARS-CoV-2 Spike Binding Clickmers.

Chembiochem : a European journal of chemical biology·2026

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Background:

  • Aptamers are crucial for targeting specific cell subpopulations in research and therapeutics.
  • Existing cell-selection methods can suffer from false positives due to non-specific binding or cell uptake.

Purpose of the Study:

  • To develop a modular and generally applicable protocol for selecting aptamers against specific cell subpopulations.
  • To provide a robust method for identifying cell-selective aptamers with reduced false positives.

Main Methods:

  • Utilizing fluorescence-activated cell sorting (FACS) to differentiate and separate cells based on aptamer binding.
  • Implementing a systematic evolution of ligands by exponential amplification (SELEX) procedure.
  • Detailed protocol includes pre-SELEX optimization and final library enrichment.

More Related Videos

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
08:01

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Published on: November 4, 2016

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
15:29

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Published on: July 10, 2010

Related Experiment Videos

Last Updated: Jun 6, 2026

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
10:46

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
08:01

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Published on: November 4, 2016

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
15:29

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Published on: July 10, 2010

Main Results:

  • The developed protocol enables the selection of aptamers with high specificity for target cell subpopulations.
  • Fluorescence-activated cell sorting minimizes false positives associated with non-specific nucleic acid binding or uptake by compromised cells.
  • Demonstrated successful application with a step-by-step protocol targeting CD19(+) Burkitt's lymphoma cells.

Conclusions:

  • This protocol offers a state-of-the-art approach for identifying aptamers that selectively target virtually any cell type.
  • The method enhances the reliability of aptamer selection for biomedical research and the development of cell-specific therapeutics.