Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 6, 2026

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
14:53

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

Quantification of nanoparticle uptake by cells using microscopical and analytical techniques.

Andreas Elsaesser1, Ashley Taylor, Gesa Staats de Yanés

  • 1Nano Systems Biology Group, Centre for Molecular Biosciences, University of Ulster, Coleraine, UK Centre for Molecular Biosciences, University of Ulster, Cromore Road, BT52 1SA, Coleraine, UK.

Nanomedicine (London, England)
|December 7, 2010
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fluorescent probes as markers of cell envelope structure and function in halophilic archaea.

Scientific reports·2026
Same author

Phase-dependent intake and 90-day mortality by nutrition status in invasive mechanical ventilation critically ill adults: A retrospective cohort study.

Asia Pacific journal of clinical nutrition·2026
Same author

Comprehensive behavioral profiling in male spontaneously hypertensive rats: latent trait mapping supports a valid multidomain ADHD model.

Translational psychiatry·2026
Same author

Public Health Concerns at the Intersection of Drinking Water Contamination and Low Healthcare Access in Maine.

Maine policy review·2026
Same author

Survival of NASA-cleanroom microbial isolates under simulated space and Martian conditions.

Applied and environmental microbiology·2026
Same author

The development of a model of acculturation and health practices among sub-Saharan African Men (MAPS) in the United States: a grounded theory study of prostate cancer early detection.

BMC public health·2026

Accurate nanoparticle quantification in biological systems is crucial for nanotoxicology and nanomedicine. Electron microscopy combined with stereology offers a reliable method for quantifying nanoparticle uptake in cells.

Area of Science:

  • Nanotoxicology
  • Nanomedicine
  • Biological Sciences

Background:

  • Quantifying nanoparticles in biological systems is essential for assessing nanomaterial behavior and risks.
  • Existing methods for nanoparticle quantification in cells and tissues require further development for reliability and comparability.
  • Microscopy and analytical techniques are used, but often lack spatial resolution or require particle labeling.

Purpose of the Study:

  • To highlight the importance of accurate nanoparticle quantification in biological systems.
  • To review current techniques for nanoparticle detection and quantification.
  • To emphasize the need for advanced methods for reliable and spatially resolved measurements.

Main Methods:

  • Review of existing microscopy techniques (light and electron microscopy) for nanoparticle detection.

More Related Videos

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry
18:07

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Published on: June 8, 2012

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles
09:19

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Published on: March 28, 2021

Related Experiment Videos

Last Updated: Jun 6, 2026

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
14:53

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry
18:07

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Published on: June 8, 2012

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles
09:19

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Published on: March 28, 2021

  • Discussion of analytical techniques like mass spectrometry.
  • Introduction of stereology as a sampling strategy for quantitative microscopy.

    • Main Results:

    • Electron microscopy provides the necessary resolution to image individual nanoparticles and determine cellular accumulation.
    • Super-resolution light microscopy is emerging as a viable option for imaging individual nanoparticles.
    • Stereology, when combined with electron microscopy, is a successful strategy for quantifying nanoparticle uptake.

    Conclusions:

    • Accurate quantification of nanoparticles in cells and tissues is vital for nanotoxicology and nanomedicine.
    • Electron microscopy offers superior resolution for individual particle imaging and localization.
    • Stereology combined with electron microscopy provides a robust method for quantitative analysis of nanoparticle uptake.