Related Experiment Video
Updated: Jun 6, 2026

Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers
Published on: December 18, 2013
An update: salivary hormones and physical exercise
1U.O. of Endocrinology, Department of Surgery and Medical Sciences, University of Padova (I), Padova, Italy.
Saliva analysis offers a non-invasive method to assess athletes' physiological stress, with salivary cortisol levels correlating to psychological indicators and anxiety during exercise. This highlights saliva as a viable alternative to serum for monitoring steroid hormones in sports science.
Area of Science:
- Sports Science and Exercise Physiology
- Endocrinology
- Biochemistry
Background:
- Saliva contains diverse biomolecules, including inorganic, organic, protein, and lipid compounds.
- Hormones like steroids, typically measured in plasma, are also detectable in oral fluid.
- The accessibility and hormonal characteristics of saliva make it a potential alternative to serum for physiological monitoring.
Purpose of the Study:
- To review the relationship between salivary steroids and psycho-physiological stress in athletes.
- To explore the utility of salivary cortisol as an indicator of psychological state and anxiety related to exercise performance.
- To examine the correlation between salivary and plasma steroid hormone levels during physical activity.
Main Methods:
- Review of existing studies focusing on salivary hormone measurements, primarily steroids, in the context of physical exercise.
- Analysis of correlations between salivary and plasma levels of cortisol, testosterone, and dehydroepiandrosterone (DHEA) during exercise.
- Consideration of how exercise type, duration, intensity, and training conditions affect salivary steroid concentrations.
Main Results:
- Salivary cortisol measurements can serve as a self-report indicator of psychological state and anxiety changes associated with exercise performance.
- Salivary and plasma steroid hormone levels (cortisol, testosterone, DHEA) show correlation during exercise.
- Exercise parameters (type, duration, intensity) and training conditions significantly influence salivary steroid concentrations, mirroring serum variations.
Conclusions:
- Saliva is a promising alternative to serum for monitoring steroid hormone responses to exercise and stress in athletes.
- Measurement of salivary steroids provides valuable insights into the psycho-physiological stress experienced by athletes.
- The use of saliva in sports disciplines for hormonal analysis represents a significant future perspective.
Related Concept Videos
Salivary Glands and Saliva
Exercise and Cardiovascular Response
Light to moderate physical activity initiates a series of interconnected responses in the body. The heart rate modestly increases in anticipation of the workout, followed by widespread vasodilation as oxygen consumption by skeletal muscles increases. This results in decreased peripheral resistance, increased capillary blood flow, and accelerated...
Sympathetic Activation
Hypothalamic-Pituitary Axis
Regulation of Hormone Secretion
Humoral stimuli,...
Hormonal Regulation of Blood Pressure
Epinephrine and Norepinephrine
The adrenal medulla releases epinephrine and norepinephrine, catecholamines that enhance and extend the sympathetic or "fight or flight" physiological response. These hormones escalate heart rate and the force of contraction while...
