Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 6, 2026

Bilateral Common Carotid Artery Occlusion as an Adequate Preconditioning Stimulus to Induce Early Ischemic Tolerance to Focal Cerebral Ischemia
07:46

Bilateral Common Carotid Artery Occlusion as an Adequate Preconditioning Stimulus to Induce Early Ischemic Tolerance to Focal Cerebral Ischemia

Published on: May 9, 2013

STAT subtype specificity and ischemic preconditioning in mice: is STAT-3 enough?

Michael D Goodman1, Sheryl E Koch, Muhammad R Afzal

  • 1Department of Surgery, University of Cincinnati, Cincinnati, OH, USA.

American Journal of Physiology. Heart and Circulatory Physiology
|December 7, 2010
PubMed
Summary

Signal transducer and activator of transcription 3 (STAT-3) deletion in the heart impairs ischemic preconditioning effectiveness. Alternative STAT subtypes (STAT-5 and -6) do not compensate for STAT-3 loss in protecting against ischemia-reperfusion injury.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Point of Care UCHL1/GFAP for Rapid Assessment of Undifferentiated Trauma Patients.

Journal of the American College of Surgeons·2026
Same author

A Contemporary Review of Subcutaneous and Extravascular Implantable Cardiac Defibrillators.

The Journal of innovations in cardiac rhythm management·2026
Same author

Modulating Cardiac-Gut Microbiome Interaction Post-Myocardial Infarction with Engineered Bacteria.

bioRxiv : the preprint server for biology·2026
Same author

Prehospital Resuscitation with Type O Whole Blood for Trauma and Hemorrhage.

The New England journal of medicine·2026
Same author

Impact of Heating Inspired Gases During Mechanical Ventilation on Resolution of Hypothermia in a Porcine Model.

Respiratory care·2026
Same author

Microvesicles in stored human whole blood increase thrombin generation via phosphatidylserine.

The journal of trauma and acute care surgery·2026

Area of Science:

  • Cardiovascular Biology
  • Molecular Cardiology
  • Signal Transduction

Background:

  • The role of Signal Transducer and Activator of Transcription (STAT) subtypes in myocardial ischemic tolerance is not fully understood.
  • STAT-3 is implicated in cellular responses to stress, but its specific role in ischemic preconditioning (IPC) remains unclear.

Purpose of the Study:

  • To investigate whether alternative STAT subtypes (STAT-5 and STAT-6) can confer myocardial protection against ischemia-reperfusion (I/R) injury in the absence of STAT-3.
  • To determine if STAT-3 deletion affects the efficacy of IPC in preserving cardiac function.

Main Methods:

  • Utilized wild-type (WT) and cardiomyocyte-specific STAT-3 knockout (KO) mice.
  • Assessed cardiac function using echocardiography and Langendorff ex vivo perfusion systems.

More Related Videos

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model
06:28

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

Published on: January 6, 2011

Surgical Approach for Middle Cerebral Artery Occlusion and Reperfusion Induced Stroke in Mice
10:30

Surgical Approach for Middle Cerebral Artery Occlusion and Reperfusion Induced Stroke in Mice

Published on: October 20, 2016

Related Experiment Videos

Last Updated: Jun 6, 2026

Bilateral Common Carotid Artery Occlusion as an Adequate Preconditioning Stimulus to Induce Early Ischemic Tolerance to Focal Cerebral Ischemia
07:46

Bilateral Common Carotid Artery Occlusion as an Adequate Preconditioning Stimulus to Induce Early Ischemic Tolerance to Focal Cerebral Ischemia

Published on: May 9, 2013

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model
06:28

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

Published on: January 6, 2011

Surgical Approach for Middle Cerebral Artery Occlusion and Reperfusion Induced Stroke in Mice
10:30

Surgical Approach for Middle Cerebral Artery Occlusion and Reperfusion Induced Stroke in Mice

Published on: October 20, 2016

  • Subjected hearts to IPC followed by I/R, analyzing STAT-5 and STAT-6 phosphorylation via Western blot.

    • Main Results:

    • Cardiac function was preserved at baseline in STAT-3 KO hearts compared to WT.
    • STAT-3 deletion significantly attenuated the protective effects of IPC on cardiac function recovery post-I/R.
    • Increased phosphorylation of STAT-5 and STAT-6 was observed in KO hearts after ischemic challenge, but this did not improve IPC efficacy.

    Conclusions:

    • Cardiac-restricted STAT-3 deletion does not affect baseline cardiac performance but impairs the effectiveness of IPC.
    • STAT-3 deletion is not associated with compensatory upregulation of STAT-5 or STAT-6 that could protect against I/R injury.
    • JAK-STAT signaling through STAT-3 is crucial for effective ischemic preconditioning and myocardial protection.