Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Peroxisomes

Peroxisomes

Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...

Peroxisomes

Peroxisomes

Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...

Protein Import into the Peroxisomes

Protein Import into the Peroxisomes

Cells contain membrane-bound organelles called peroxisomes that oxidize organic molecules by transferring hydrogen atoms to oxygen, producing hydrogen peroxide. Peroxisomes enzymatically convert the released hydrogen peroxide into water and oxygen.
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

<i>Salmonella</i> Typhi asparaginase-dependent activation of GCN2 promotes bacterial killing in murine macrophages.

Infection and immunity·2026

Same author

Reconstructing EBV reactivation and DNA damage response kinetics in morphologic pseudotime.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Nanoscale Direct-to-Biology Optimization of Cdk2 Inhibitors.

Journal of medicinal chemistry·2026

Same author

Comprehensive Proteomic Profiling Reveals Dysregulation of Angiogenesis and Inflammatory Pathways in the Brains of SIRT3 Knockout Mice.

Brain sciences·2026

Same author

BRD4-mediated ER membrane contact creates functionally distinct mitochondrial subtypes.

Molecular cell·2026

Same author

Identification and characterization of the functional LolB ortholog in <i>Bacteroides</i>.

bioRxiv : the preprint server for biology·2026

Search research articles

Related Experiment Video

Updated: Jun 6, 2026

Peroxisome Staining in Mammalian Cells Using Peroxisome-Specific Probes

Peroxisome Staining in Mammalian Cells Using Peroxisome-Specific Probes

Published on: December 19, 2025

High content screening for non-classical peroxisome proliferators.

Jonathan Z Sexton¹, Qingping He, Lawrence J Forsberg

¹Biomanufacturing Research Institute and Technology Enterprise (BRITE), North Carolina Central University.

International Journal of High Throughput Screening

|December 7, 2010

Summary

This summary is machine-generated.

Scientists developed a new screening method to find compounds that increase peroxisome function in human cells, potentially aiding metabolic syndrome and type 2 diabetes treatments.

More Related Videos

Monitoring Stub1-Mediated Pexophagy

Monitoring Stub1-Mediated Pexophagy

Published on: May 12, 2023

Related Experiment Videos

Last Updated: Jun 6, 2026

Peroxisome Staining in Mammalian Cells Using Peroxisome-Specific Probes

Peroxisome Staining in Mammalian Cells Using Peroxisome-Specific Probes

Published on: December 19, 2025

Monitoring Stub1-Mediated Pexophagy

Monitoring Stub1-Mediated Pexophagy

Published on: May 12, 2023

Area of Science:

Cell Biology
Biochemistry
Drug Discovery

Background:

Peroxisomes are crucial organelles involved in metabolic processes and detoxification.
Rodent studies show peroxisome proliferators can treat metabolic syndrome (MetS) and type 2 diabetes (T2D), but human responses differ.
Existing methods to identify human peroxisome modulators are limited.

Purpose of the Study:

To develop and validate a novel high-throughput screening (HTS) assay for identifying compounds that induce peroxisome biogenesis in human cells.
To discover new small molecules capable of modulating peroxisome function for potential therapeutic applications.

Main Methods:

Designed a genetically encoded, high-content screening assay for human cells.
Validated the assay using 4-phenylbutyrate (PBA) as a positive control, confirming its PPAR-independent peroxisome proliferator activity.
Screened a library of 15,000 compounds, achieving a Z' factor of 0.74.

Main Results:

Identified 4 existing drugs and 10 novel compounds that increase peroxisome proliferation in human cells.
Discovered novel compounds with common scaffolds exhibiting up to 1000-fold greater potency than PBA.
The screening assay proved effective for identifying peroxisome modulator compounds.

Conclusions:

A robust HTS assay for identifying human peroxisome proliferators has been established.
Novel drug candidates with significant potency have been identified.
These findings offer potential scaffolds for developing new treatments for MetS/T2D.