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Related Experiment Video

Updated: Jun 5, 2026

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
09:14

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Published on: September 28, 2022

Reconstructing EBV reactivation and DNA damage response kinetics in morphologic pseudotime.

Dina G Tekle1, Craig J Dobry2, Jonathan Z Sexton3

  • 1Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109.

Proceedings of the National Academy of Sciences of the United States of America
|June 3, 2026
PubMed
Summary
This summary is machine-generated.

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Epstein-Barr Virus Infection at Single-Cell Resolution.

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Phenotypic Screening Coupled with AI-Driven Target Deconvolution Identifies α-Terthienyl as a Dual DPP-IV/HSD17β13 Modulator with Efficacy in a Mouse Model of MASLD.

bioRxiv : the preprint server for biology·2025

Epstein-Barr virus (EBV) subverts host DNA damage responses (DDR) during lytic infection. This study reveals EBV transiently uses DDR for replication, but impairs host DDR, impacting oncogenesis and autoimmunity.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Epstein-Barr virus (EBV) lytic infection is linked to cancer and autoimmune diseases.
  • EBV's lytic cycle relies on manipulating host DNA damage responses (DDR).

Purpose of the Study:

  • To systematically analyze EBV reactivation and DDR dynamics at the single-cell level.
  • To create a comprehensive atlas of cellular phenotypes during EBV lytic infection.

Main Methods:

  • High-content screening (HCS) was used to capture cell morphology and dynamics.
  • Pseudotemporal analysis was applied to >850,000 cells to track EBV reactivation and DDR.
  • Cell segmentation and feature quantification identified distinct cellular responses.

Main Results:

Keywords:
B cell lymphomaDNA damageEpstein-Barr virushigh-content screeninghost-virus interactions

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Related Experiment Videos

Last Updated: Jun 5, 2026

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
09:14

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Published on: September 28, 2022

An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus
09:53

An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus

Published on: August 25, 2017

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
06:38

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Published on: November 8, 2011

  • Generated a large-scale cell atlas detailing EBV lytic protein and DDR factor localization.
  • Observed distinct DDR profiles in lytic versus latent cells and varied protein localization with stimuli.
  • Discovered that early DDR marker γH2AX was depleted from viral replication compartments, while 53BP1 was transiently present then absent.

Conclusions:

  • EBV appears to transiently utilize host DNA double-strand break (DSB) DDR proteins to initiate viral genome replication.
  • Host-targeted DDR is initiated but ultimately impaired during EBV reactivation.
  • HCS combined with pseudotime analysis offers a powerful method for studying host-virus interactions.