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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
DNA-templated covalent coupling of G4 PAMAM dendrimers
Huajie Liu1, Thomas Tørring, Mingdong Dong
1Centre for DNA Nanotechnology (CDNA) at The Interdisciplinary Nanoscience Center (iNANO), Aarhus University, DK-8000 Aarhus C, Denmark.
Abstract:
Generation-4 polyamidoamine (PAMAM) dendrimers were surface-functionalized with azides or alkynes and conjugated to one DNA strand. DNA-controlled self-assembly of alternating azide and alkyne dendrimers on a DNA template enabled the coupling of the dendrimers by the azide-alkyne "click" reaction to form covalently coupled dimers, trimers, and tetramers. Polymerization of the DNA-dendrimer conjugates was also demonstrated, as well as assembly in a circular structure on DNA origami and imaging by atomic force microscopy.
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