Related Experiment Video
Updated: Jun 6, 2026
10:26
Inducing Apical Periodontitis in Mice
Published on: August 6, 2019
TEGDMA reduces mineralization in dental pulp cells
K M Galler1, H Schweikl, K-A Hiller
1Department of Operative Dentistry and Periodontology, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93042 Regensburg, Germany.
Journal of Dental Research
|December 8, 2010
Summary
Triethylene glycol dimethacrylate (TEGDMA) in dental bonding agents may hinder reparative dentin formation. Studies show TEGDMA inhibits mineralization in human pulp cells, impacting healing after dental procedures.
Area of Science:
- Biomaterials Science
- Dental Research
- Cell Biology
Background:
- Direct pulp capping with dentin bonding agents is a common dental procedure.
- However, in vivo studies show limited success in forming reparative dentin.
Purpose of the Study:
- To investigate the role of triethylene glycol dimethacrylate (TEGDMA), a component of dentin bonding agents, in inhibiting mineralization.
- To assess the impact of TEGDMA on human pulp cells and their ability to form reparative dentin.
Main Methods:
- Human pulp cells were exposed to varying concentrations of TEGDMA.
- Gene expression of mineralization markers (collagen I, alkaline phosphatase, bone sialoprotein, osteocalcin, Runx2, dentin sialophosphoprotein) was analyzed using real-time PCR.
- Alkaline phosphatase activity and calcium deposition were measured over 14 days.
Main Results:
- TEGDMA exposure led to a concentration- and time-dependent decrease in mineralization markers.
- A subtoxic TEGDMA concentration (0.3 mM) significantly reduced gene expression and alkaline phosphatase activity.
- Calcium deposition was also significantly lower in TEGDMA-treated cells.
Conclusions:
- Low concentrations of TEGDMA can inhibit mineralization in dental pulp cells.
- This inhibition may impair reparative dentin formation following pulp capping with dentin bonding agents containing TEGDMA.
