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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 6, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Sequential array cytometry: multi-parameter imaging with a single fluorescent channel.

Daniel R Gossett¹, Westbrook M Weaver, Noor S Ahmed

¹Department of Bioengineering, University of California Los Angeles, CA, USA.

Annals of Biomedical Engineering

|December 8, 2010

Summary

This summary is machine-generated.

This study presents a new single-cell analysis platform for personalized medicine. It uses a novel method to analyze intracellular components, enabling better disease diagnosis and treatment strategies.

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Area of Science:

Biomedical Engineering
Cell Biology
Analytical Chemistry

Background:

Human population and tissue heterogeneity necessitate personalized medicine.
Single-cell functional assays are crucial for identifying cellular heterogeneity and guiding treatment decisions.
Existing methods face challenges with overlapping fluorescence spectra and high costs.

Purpose of the Study:

To develop a high-throughput, single-cell analysis platform for personalized diagnostics.
To enable precise cell and fluid handling using hydrodynamic arrays.
To overcome limitations of spectral overlap and reduce costs in multi-analyte single-cell assays.

Main Methods:

Utilizing well-characterized hydrodynamic cell isolation arrays for precise cell handling.
Employing a single fluorescent channel for sequential staining of intracellular components.
Integrating lens-free fluorescent imaging, fiber-optic scanning, and microlens arrays.
Analyzing DNA content, nucleus-to-cytoplasm ratio, and surface protein glycosylation.
Measuring temporal localization of cellular components and intracellular reaction kinetics in real-time.

Main Results:

Demonstrated extraction of spatial and temporal information using a single fluorescent channel.
Successfully analyzed DNA amount, nucleus-to-cytoplasm ratio, and protein glycosylation.
Enabled real-time measurements of cellular component localization and intracellular reaction kinetics.
Showcased the potential for measuring multi-drug resistance through reaction kinetics.
Validated the platform's efficacy for advanced single-cell assays.

Conclusions:

The developed platform offers a cost-effective and efficient approach for single-cell analysis.
This technology facilitates the uncovering of cellular heterogeneity for personalized medicine.
It is a foundational step towards improved diagnostics and personalized treatments for complex diseases.