Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Glycosylation

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

Proteoglycans

Proteoglycans

Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...

Pharmacogenomics: Identification of New Drug Targets

Pharmacogenomics: Identification of New Drug Targets

Advances in genomics have profoundly influenced drug discovery by increasing both the speed and accuracy of pharmaceutical development. Pharmacogenomics, which examines how genetic variation influences drug response, facilitates the identification of novel therapeutic targets and enables patient stratification for personalized treatment. These strategies contribute to improved drug efficacy, minimized adverse effects, and more efficient clinical trial design.Mapping genetic differences...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

mTORC1 signaling requires proteasomal function and the involvement of CUL4-DDB1 ubiquitin E3 ligase.

Cell cycle (Georgetown, Tex.)·2008

Same author

Prospective study of liver transplant recipients with HCV infection: evidence for a causal relationship between HCV and insulin resistance.

Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·2008

Same author

Quantitative gel electrophoresis: sources of variation.

Journal of proteome research·2008

Same author

Evidence that the Nijmegen breakage syndrome protein, an early sensor of double-strand DNA breaks (DSB), is involved in HIV-1 post-integration repair by recruiting the ataxia telangiectasia-mutated kinase in a process similar to, but distinct from, cellular DSB repair.

Virology journal·2008

Same author

[Inhibitory effects of Qushi Huayu Decoction on fatty deposition and tumor necrosis factor alpha secretion in HepG2 cells induced by free fatty acid].

Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine·2008

Same author

Bioactive polybrominated diphenyl ethers from the marine sponge Dysidea sp.

Journal of natural products·2008

Search research articles

Related Experiment Video

Updated: Jun 6, 2026

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

Glycoproteomics and clinical applications.

Yuan Tian¹, Hui Zhang

¹Department of Pathology, Johns Hopkins University, Baltimore, MD, USA.

Proteomics. Clinical Applications

|December 8, 2010

Summary

Glycoproteomics analyzes proteins with attached carbohydrates, revealing their roles in health and disease. Recent advancements in glycoproteomic methods enhance understanding of protein glycosylation and its clinical applications.

Area of Science:

Biochemistry and Molecular Biology
Proteomics
Glycobiology

Background:

Protein glycosylation is a complex and vital post-translational modification involved in numerous biological processes.
Aberrant glycosylation is implicated in the pathogenesis and progression of various diseases.
Glycoproteomics provides tools to study the structure, function, and abundance of glycoproteins.

Purpose of the Study:

To review recent advancements in glycoproteomic analysis methods.
To highlight the importance of these techniques in understanding the link between protein glycosylation and disease.
To discuss the clinical applications of glycoproteomic approaches.

Main Methods:

Enrichment strategies for glycoproteins with specific carbohydrate structures.

More Related Videos

Glycopeptide Capture for Cell Surface Proteomics

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

Related Experiment Videos

Last Updated: Jun 6, 2026

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

Glycopeptide Capture for Cell Surface Proteomics

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

Quantitative proteomic analysis of enriched glycoproteins.

Characterization of glycosylation sites and carbohydrate structures.

Main Results:

Glycoproteomic studies can identify glycosylated proteins, determine glycosylation sites, and characterize carbohydrate structures.
These studies reveal the abundance and function of glycoproteins in biological and pathological contexts.
Recent method developments are crucial for elucidating the relationship between glycosylation and disease.

Conclusions:

Advanced glycoproteomic techniques are essential for understanding the role of protein glycosylation in disease.
The clinical applications of glycoproteomic methods are expanding.
Further development in glycoproteomics will deepen insights into disease mechanisms and therapeutic strategies.