Efficient conjugation doxorubicin to bacterial magnetic nanoparticles via a triplex hands coupling reagent
Lin Guo1, Ji Huang, Li-Min Zheng
1Key Laboratory of Mesoscopic Chemistry of MOE and School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
Journal of Nanoscience and Nanotechnology
|December 9, 2010
Abstract:
By using a novel triplex "hand" coupling reagent, tris-succinimidyl aminotriacetate (TSAT), doxorubicin loading efficiency of BMP can be greatly enhanced in comparison with that of dual functional linkers. The drug loading ratio of doxorubicin-coupled bacterial magnetic nanoparticles produced by this method (DBMP-T) is 62%. DBMP-T disperse well, have a narrow size distribution, exhibit a high magnetic response, is sensitive to pH to facilitate drug release and are strongly cytotoxic to HePG2 and MCF-7 cancer cells.
