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Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Jun 6, 2026

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis
05:47

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis

Published on: April 4, 2025

Planar patch clamp approach to characterize ionic currents from intact lysosomes.

Michael Schieder1, Katrin Rötzer, Andrea Brüggemann

  • 1Center for Integrated Protein Science CIPS-M and Zentrum für Pharmaforschung-Department Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5 -13, D-81377 München, Germany.

Science Signaling
|December 9, 2010
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to study intracellular ion channels using patch clamp electrophysiology on immobilized organelles. This technique successfully recorded currents from lysosomes, overcoming previous limitations with fragile membranes.

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Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

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Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis
05:47

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Published on: April 4, 2025

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Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
15:28

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Published on: October 1, 2010

Area of Science:

  • Cell Biology
  • Biophysics
  • Electrophysiology

Background:

  • The patch clamp technique is vital for studying ion channels in the plasma membrane.
  • Its application to intracellular ion channels is limited due to the fragility of organelle membranes.

Purpose of the Study:

  • To develop a novel method for patch clamp electrophysiology of intracellular ion channels.
  • To enable stable recordings from delicate organelle membranes.

Main Methods:

  • Immobilization of isolated organelles onto a solid matrix planar glass chip.
  • Utilizing a microstructured hole in the chip to facilitate gigaseal formation and electrophysiological recordings.
  • Using lysosomes as a model system for method development.

Main Results:

  • Successfully recorded endogenous ionic currents from wild-type lysosomes.
  • Demonstrated recordings from lysosomes engineered to overexpress specific ion channels.
  • Validated the method's ability to maintain seal integrity with fragile intracellular membranes.

Conclusions:

  • The developed method overcomes limitations of traditional patch clamp for intracellular studies.
  • This technique provides electrophysiological access to a wide array of intracellular ion channels.
  • Opens new avenues for investigating organelle function and ion transport.