Related Experiment Video
Updated: Jun 6, 2026
Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography
Published on: May 19, 2022
Cloning, expression, purification, crystallization and preliminary X-ray studies of a secreted lectin (Rv1419) from
Dhabaleswar Patra1, R Srikalaivani, Ashish Misra
1Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India.
Abstract:
A secreted lectin, Rv1419, from Mycobacterium tuberculosis has been cloned, expressed, purified and crystallized and the crystals have been characterized. This represents the first X-ray investigation of a lectin or lectin-like molecule from the pathogen. The cubic crystals contain one molecule in the asymmetric unit. Sequence comparisons indicate that the lectin has a β-trefoil fold and belongs to a well characterized family of carbohydrate-binding modules. Structural analysis of the crystals is in progress.
