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Tumor Treating Field Therapy in Combination with Bevacizumab for the Treatment of Recurrent Glioblastoma
Published on: October 27, 2014
Bevacizumab therapy normalizes the pathological intraocular environment beyond neutralizing VEGF
Rajesh K Sharma1, Anna T Rogojina, K V Chalam
1Department of Ophthalmology, University of Florida, College of Medicine, Jacksonville FL, USA. rksharma10101@gmail.com
Molecular Vision
|December 9, 2010
Summary
Intravitreal bevacizumab injections normalize the ocular environment in VEGF-related diseases beyond just blocking VEGF. A systems approach reveals broad physiological improvements after treatment.
Area of Science:
- Ophthalmology
- Molecular Biology
- Systems Biology
Background:
- Vascular endothelial growth factor (VEGF) drives neovascularization in ocular diseases.
- Anti-VEGF therapy, like bevacizumab, targets VEGF but offers a limited view of disease pathobiology.
- A systems approach is needed for an integrated understanding of VEGF-related ocular conditions.
Purpose of the Study:
- To investigate the global effects of intravitreal bevacizumab on ocular physiology.
- To determine if bevacizumab normalizes the perturbed ocular environment in VEGF-related diseases.
Main Methods:
- Analysis of 90 aqueous humor analytes from 8 patients before and after intravitreal bevacizumab injections.
- Utilized Luminex xMAP microbead technology for large-scale analyte detection.
- Compared pre- and post-injection analyte levels against known normal values.
Main Results:
- 43 analytes were detected, including VEGF, C-reactive protein, glutathione, and cytokines.
- 24 markers showed significant perturbation (>1.5 fold) in pre-injection samples compared to normal.
- Post-injection samples exhibited a clear trend toward normalization of these perturbed analytes.
Conclusions:
- Intraocular bevacizumab injections promote normalization of the ocular physiological environment.
- Bevacizumab's therapeutic effects extend beyond VEGF neutralization.
- Large-scale aqueous humor analysis via a systems approach offers valuable insights into ocular diseases and treatment responses.
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