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Androgen binding sites in human temporal cortex.

A Sarrieau1, J B Mitchell, S Lal

  • 1Douglas Hospital Research Centre, Department of Psychiatry, Montreal, Canada.

Neuroendocrinology
|June 1, 1990
PubMed
Summary
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Researchers found androgen receptors in human brain cortex, similar to those in other mammals. This suggests androgens may influence brain activity in humans.

Area of Science:

  • Neuroendocrinology
  • Molecular Neuroscience
  • Human Brain Research

Background:

  • Androgen receptors (AR) are crucial for mediating the effects of androgens in the body.
  • Previous studies have identified AR in the central nervous system (CNS) of rodents and non-human primates.
  • The presence and characteristics of AR in the human cortex remained largely uncharacterized.

Purpose of the Study:

  • To investigate the presence and pharmacological properties of androgen receptor binding sites in the human temporal cortex.
  • To determine if human cortical AR are similar to those found in other species' CNS.

Main Methods:

  • Utilized in vitro exchange assays with soluble fractions from human temporal cortex biopsy samples.
  • Employed [3H]5 alpha-dihydrotestosterone as a radioligand to detect and quantify AR binding.

Related Experiment Videos

  • Conducted competition studies using various androgens to characterize binding specificity.

    • Main Results:

    • Demonstrated highly specific and saturable binding of [3H]5 alpha-dihydrotestosterone, indicating the presence of AR.
    • Determined an apparent affinity constant (Kd) of approximately 2 nM and a binding capacity (Bmax) of approximately 50 fmol/mg protein.
    • Found that only testosterone and 5 alpha-dihydrotestosterone effectively competed for binding sites, similar to rodent and primate CNS.

    Conclusions:

    • Confirmed the presence of androgen receptors in the human cerebral cortex.
    • The pharmacological profile of human cortical AR is highly similar to those in rodent and non-human primate CNS.
    • Suggests a potential mechanism for circulating androgens to influence neuronal activity within the human cortex.