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Rapid confirmatory identification of Mycobacterium bovis using a dot blotting immunodetection technique
G M Veerman1, R Kelman, J Colley
1Department of Primary Industries, Animal Research Institute, Yeerongpilly, Brisbane, Qld., Australia.
Veterinary Microbiology
|May 1, 1990
Abstract:
A safe, cost effective and accurate immunodetection technique for the rapid identification of Mycobacterium bovis colonies is described. One hundred and thirteen M. bovis isolates were differentiated from other species of mycobacteria using a M. bovis specific monoclonal antibody, with nitrocellulose membrane as the solid support. The technique confirms M. bovis identification in only 4 h compared to 3 weeks for conventional antibiotic sensitivity tests.