[Methodological approaches to evaluation of prostaglandin synthesis by isolated human thrombocytes]

Individual steps of prostaglandin-synthetase activity evaluation in human platelets are considered in detail, involving isolation of platelets, incubation of washed cells with labelled arachidonic or dihomo-gamma-linolenic acids, extraction of prostaglandins from incubation medium and their separation as well as quantitative estimation of the prostaglandins developed (nmoles/10(9) platelets/10 min). The methodical approaches suggested enabled not only to avoid some errors during estimation of the prostaglandin-synthetase activity in individual platelets but also to increase accuracy, sensitivity and specificity of the procedure as compared with similar methods.