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Quantitative analysis of closantel and rafoxanide in bovine and ovine muscles by high-performance liquid
Hoi-Sze Yeung1, Wing-Hong Ching, Shirley Sau-Ling Lai
1Analytical and Advisory Services Division, Government Laboratory, 7/F Ho Man Tin Government Office, Ho Man Tin, Kowloon, Hong Kong Special Administrative Region, China.
Abstract:
An HPLC method with a fluorescence detector (HPLC-FLD) was described for the quantitative determination of closantel and rafoxanide in bovine and ovine muscles. A structural analog closely related to rafoxanide, viz., N-[4-(4-chlorophenoxy)phenyl]-2-hydroxy-3,5-diiodobenzamide, was synthesized as an internal standard. Bovine and ovine muscles were extracted with acetonitrile-acetone (60 + 40, v/v) followed by cleanup on mixed mode anionic exchange SPE cartridges. After evaporation and reconstitution with the mobile phase, the sample was analyzed by HPLC-FLD using internal standard calibration. The method was validated by using fortified bovine and ovine muscles at 15, 30, and 60 microg/kg. The accuracy and RSD were 70-110% and < or =10%, respectively.
