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Updated: Jun 6, 2026

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
Published on: January 10, 2011
[Actions of Syn-1A on blocking the activation of K(ATP) channel induced by acidic pH]
Jun-Li Ma1, Guo-Qing Zhuang, Yan-Xia Luo
1School of Public Health and Family Medicine, Capital Medical University, Beijing 100069, China.
Aim:
To investigate the action and mechanism of Syn-1A in reversing the activation of K(ATP) channel induced by weak acidic pH.
Methods:
The patches excised from Kir6.2/SUR2A expressing HEK-293 cells were used to establish inside-out configuration. To examine the actions of weak acidic pH in activation of the channel and the reverse action of Syn-1A on it, the inside-out patches were continuously perfused with the solution of pH from 7.4, 7.0, 6.8, 6.5 to 6.0 with or without Syn-1A. In vitro binding was employed to study the influence of different pH to the binding of Syn-1A to SUR2A subunit.
Results:
Syn-1A blocked pH 6.5, 6.8 and 7.0 induced activation of the channel, and Syn-1A binding to SUR2A were increased by reducing pH from 7.4 to 6.0.
Conclusion:
Syn-1A would assert some inhibition of the KATP channels, which might temper the fluctuation of acidic pH-induced K(ATP) channel opening that could induce fatal re-entrant arrhythmias.
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