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[Anti-hypoxia protective role of the effective component extracted from angelia injection]
Ming-Ming Li1, Li-Ying Wu, Tong Zhao
1Institute of Neuro-Science, Capital Medical University School of Basic Medical Sciences,Beijing 100069, China.
Aim:
To investigate anti-hypoxia protective roles of the effective component extracted from angelia injection using hypoxia injury model in mice and ECV304 cells separately.
Methods:
The survival time of mice was observed separately under normobaric and hypobaric hypoxia. The activity of ECV304 cells was tested by MTT assay, and the mortality rate was examined by Trypan blue exclusion assay to evaluate the pharmacodynamic effects.
Results:
After exposed to hypoxia the survival time of mice was increased in medicine groups,compared with the control groups (P < 0.05). The cell survival rate was decreased and the cell mortality rate was increased after cells were exposed to hypoxia,while the cell survival rate was significantly increased (P < 0.01), and the cell mortality rate was significantly decreased (P < 0.1) in the medicine groups compared with the control groups.
Conclusion:
The effective component extracted from angelia injection can protect against the injury induced by hypoxia.
Insights
The effective component from angelica injection enhances survival against hypoxia. This study demonstrates its protective effects in both animal models and cell cultures, highlighting its potential therapeutic benefits.
Area of Science:
- Pharmacology
- Cell Biology
- Physiology
Background:
- Hypoxia poses a significant threat to cellular and organismal survival.
- Identifying protective agents against hypoxia is crucial for developing effective medical interventions.
Purpose of the Study:
- To evaluate the anti-hypoxia protective effects of an effective component from angelica injection.
- To investigate its efficacy in both in vivo (mice) and in vitro (ECV304 cells) models of hypoxia-induced injury.
Main Methods:
- Mice were subjected to normobaric and hypobaric hypoxia to assess survival time.
- ECV304 cell viability was measured using MTT assay.
- Cellular mortality was determined via Trypan blue exclusion assay.
Main Results:
- Treatment with the angelica component significantly increased survival time in mice exposed to hypoxia (P < 0.05).
- In cell cultures, the component markedly improved cell survival rate (P < 0.01) and reduced mortality rate (P < 0.1) under hypoxic conditions.
Conclusions:
- The effective component extracted from angelica injection exhibits significant protective properties against hypoxia-induced injury.
- These findings support the therapeutic potential of this component in managing conditions related to hypoxia.
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