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Related Concept Videos

Glycocalyx and its Functions01:14

Glycocalyx and its Functions

The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
Components of...
Protein Glycosylation01:25

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...

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Updated: Jun 6, 2026

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy
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Published on: February 7, 2025

A versatile method for functionalizing surfaces with bioactive glycans.

Fang Cheng1, Jing Shang, Daniel M Ratner

  • 1Department of Bioengineering, University of Washington, Seattle, Washington 98195, United States.

Bioconjugate Chemistry
|December 15, 2010
PubMed
Summary
This summary is machine-generated.

Researchers developed a simple method to create functional glycan microarrays using divinyl sulfone (DVS) surfaces. This technique allows for the covalent immobilization of carbohydrates and glycoproteins, advancing glycomics research for nonexpert labs.

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Last Updated: Jun 6, 2026

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Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay
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Published on: July 13, 2017

Area of Science:

  • Biochemistry
  • Materials Science
  • Analytical Chemistry

Background:

  • Microarrays and biosensors require stable surface-bound biomolecules for functional assays.
  • Existing immobilization methods can be complex, limiting accessibility for some research labs.
  • High-throughput screening of molecular interactions is crucial in 'omics' research.

Purpose of the Study:

  • To demonstrate a simplified chemistry for fabricating functional glycan microarrays.
  • To utilize divinyl sulfone (DVS)-modified surfaces for covalent carbohydrate immobilization.
  • To enable nonexpert laboratories to construct glycan microarrays and biosensors.

Main Methods:

  • Covalent immobilization of natural and chemically derived carbohydrates and glycoproteins onto DVS-modified surfaces.
  • Quantitative bioactivity assessment using surface plasmon resonance imaging (SPRi).
  • Surface characterization via X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS).

Main Results:

  • Successful, straightforward, and reproducible conjugation of diverse glycans and glycoproteins onto DVS surfaces.
  • Demonstration of site-selective glycan immobilization based on nucleophilicity and anomeric configuration.
  • Quantitative bioactivity data obtained using SPRi, confirming retained function of immobilized glycans.

Conclusions:

  • The DVS-modified surface chemistry provides a facile and robust method for glycan immobilization.
  • This simplified approach significantly impacts glycomics research by enabling rapid construction of functional glycan microarrays.
  • The method expands the capabilities of nonsynthetic laboratories in quantitative biosensor development.