Related Experiment Videos
Transient induction of c-jun during hepatic regeneration
J A Alcorn1, S P Feitelberg, D A Brenner
1Department of Medicine, University of California, San Diego.
Hepatology (Baltimore, Md.)
|June 1, 1990
Summary
Cellular oncogene c-jun expression significantly increases in mouse liver after partial hepatectomy, driven by both transcriptional and posttranscriptional mechanisms. This suggests a role for c-jun in liver regeneration and extracellular matrix remodeling.
Area of Science:
- Molecular Biology
- Cellular Biology
- Oncogenes
Background:
- The cellular oncogene c-jun is transiently expressed in proliferating cells.
- c-jun has not been identified in normal liver tissue.
- Partial hepatectomy in mice triggers coordinated cell proliferation in the remaining liver.
Purpose of the Study:
- Investigate c-jun gene expression following partial hepatectomy in mice.
- Determine the cellular origin of c-jun transcripts after liver surgery.
- Elucidate the mechanisms regulating c-jun induction post-hepatectomy.
Main Methods:
- Northern blot analysis of whole liver mRNA.
- In situ hybridization and cell fractionation (parenchymal and nonparenchymal).
- Cycloheximide treatment and run-on transcriptional assays.
Main Results:
- c-jun expression increased 13-fold within 30 minutes after partial hepatectomy.
- c-jun transcripts were detected in both parenchymal and nonparenchymal liver cells.
- Hepatectomy enhanced c-jun induction independently of new protein synthesis, involving transcriptional and posttranscriptional regulation.
Conclusions:
- Partial hepatectomy induces rapid and significant c-jun expression in mouse liver.
- Both transcriptional and posttranscriptional mechanisms contribute to c-jun induction.
- c-jun may play a role in extracellular matrix remodeling to support liver cell proliferation.